We do a lot of microscopy and love a good time-lapse.
Crithidia fasciculata cells adhered to a Mattek dish and imaged by confocal microscopy for approximately 24 h.
Crithidia fasciculata cell modified to express a mitochondrial green fluorescent protein that has been fixed and stained with DAPI. Cell imaged by confocal microscopy and 3D representation and skeletonization was performed using Imaris software.
A rosette of live Crithidia fasciculata cells expressing mitoGFP and growing while adhered to a Mattek dish. Time-lapse confocal microscopy.
Another rosette of live adherent C. fasciculata cells expressing mitoGFP. Towards the end of the video the cell on the right divides.